The fellows receive a 4 month specialist training period in pediatric infectious diseases at PACMRC  and learn about conventional and advanced lab methods. Studies are entirely run and supervised by the professors and fellows spend one month in bacteriology, virology, mycology and immunology labs , each at a time.   

Virology Curriculum

The virology curriculum has been developed by Dr. M. Ziayan and consists of 25 days  of training, 4 hours a day, specially intended for fellows in pediatric infectious diseases.

 

Curriculum Coverage   

 1) Cell and  tissue  culture and isolation of viruses

A. Preparing culture media, solution and required materials

B. Learning about the studied cells ( Primary, Diploid, and immortal)

C. Cell Passage and growing

D. Storing and nourishing the cell cultures

E. Receiving  and transferring virus samples for isolation

F. Inoculation/ Transfection of virus into cells, isolation and CPE visual( microscopic)  examination

G. Cell storage in liquid nitrogen and extraction/isolation

2) Rapid diagnosis of respiratory viral infections by DIF

A. Sampling from sites, collecting and sending for lab tests

B. Preparing slides using cytospin centrifuge

C. Staining

D. Visual examination of the infected cells using a fluorescence microscope
 E. Analysis and interpretation of the results

3. Rapid quantitative diagnosis of human cytomegalovirus infection by pp65 Antigenemia Assay

A. Obtaining whole blood sample

B. Placement, wash and counting PMNs using hemocytometer

C. Preparing slides using cytopsin centrifuge 

D. Staining

E. Examination of the slides using a fluorescence microscope and learning how to view the virus infected cells  

F. Analysis and interpretation of the results

4) Using PCR to diagnose viral infective agent

A. Teaching the respective PCR theories

B. Obtaining CSF for HSV and whole blood for CMV

C. DNA extraction from the samples

D. Preparing mastermix and adding purified DNA

E. Introducing PCR system and three stage procedures ( Denaturation, Annealing, Extension)

F. Preparing gel and running the PCR  products

G. Visual examination of PCR products and preparing images/figures  and reports
5) Using Nested-RT-PCR to diagnose viral infective agent

A. Obtaining whole blood samples for Hepatitis C

B. RNA extraction from the samples

C. Preparing RT mix for converting RNA to DNA

D. Preparing mastermix and adding DNA

E. Preparing gel and running the PCR  products PCR

F. Visual examination of the products and preparing images and reports

6) Using Real-Time PCR for quantitative and qualitative diagnoses of viral infections   

A. Performing the whole test procedures forHBV, HSV or CMV

B. Learning about the features of the test

C. Sketching normal /standard graph and learning its features

D. Investigating the reliability of the test and its interpretation

E.Learning the underlying theories of Real-Time PCR methods

7) Using ELISA to detect antivirus antibodies

A. Performing the test procedures to detect  EBV anticaspid IgM

B. Visual examination and interpretation

C. Learning theoretical underpinnings of types of ELISA      

 

 

 

 




 

 

 
Shiraz University of Medical Sciences
  Prof. Alborzi Clinical Microbiology Research Center
Tel :+98713 6474296
Fax :+98713 6474303
Email : alborzicmrc@sums.ac.ir

Ph.D. by Research
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